Abstract:To elucidate the bioactive chemical components of Grifola frondosa and their antioxidant and tumor cell inhibition effects, the present study analyzed the main chemical components of ethanol extracts of Grifola frondosa extracted with various polar solvents, as well as their scavenging abilities to 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), and hydroxyl radicals. The inhibitory effects on human hepatocellular carcinoma cells (HepG2), human lung adenocarcinoma cells (A549) were also investigated. The results showed that ethyl acetate extract and n-butanol extract had the highest antioxidant activity, and the mass fractions of total phenols and flavonoids were significantly positively correlated with antioxidant activity (P<0.05). The petroleum ether extract and ethyl acetate extract showed the best inhibitory effect on tumor cells, and the mass fractions of triterpenoids and sterols showed a highly significant positive correlation with tumor cell inhibition (P<0.01). Ultrahigh performance liquid chromatography-mass spectrometry (UPLC-MS) identified a total of 15 compounds from the ethanol extracts of Grifola frondosa, among which apigenin and epicatechin might be the main chemical components responsible for the antioxidant activity of Grifola frondosa, while the steroid compounds exhibiting inhibitory effects on tumor cells might be ergosterol and ergosterol endoperoxide. The results could provide a theoretical basis for the further development of Grifola frondosa as the functional food.
