玉米糖肽的纳滤脱盐工艺

Desalination of Zein Glycopeptides via Nanofiltration Membrane

摘要:

为了去除玉米糖肽溶液中过多的盐分，采用相对分子质量200 纳滤膜对具有抗氧化活性的玉米糖肽溶液进行脱盐处理，通过对膜通量以及玉米糖肽溶液的Na ⁺质量分数、脱盐率、电导率、短肽回收率和纳滤后产物自由基清除率和Fe ²⁺ -螯合率等参数的研究，确定玉米糖肽溶液的纳滤脱盐工艺。结果表明，利用相对分子质量200纳滤膜对玉米糖肽溶液脱盐的工艺条件为：在压力2.0 MPa、温度20 ℃条件下连续纳滤5次，每次体积浓缩倍数为2。经5次纳滤后，玉米糖肽截留液的电导率降低60.87%，脱盐率和短肽回收率分别为81.60%和96.97%，羟自由基清除率从11.25%提高到15.85%，而1，1-二苯基-2-三硝基苯肼自由基清除率和Fe²⁺-螯合率分别降低11.83%和46.07%。同时，相对分子质量200纳滤膜还可以除去糖基化反应体系中55.54%未反应的D-氨基葡萄糖，起到脱盐、脱糖和浓缩的三重作用。

Abstract:

In order to remove the excessive salt from zein glycopeptide solution, the nanofiltration membrane with a molecular weight cut-off of 200 was applied for the desalination of antioxidant zein glycopeptide solution. The membrane flux, Na⁺ mass fraction, desalination rate, electrical conductivity, recovery rate of short peptide of zein glycopeptide solution, and the free radical scavenging rate and Fe²⁺-chelating activity of products after nanofiltration were studied to determine the nanofiltration desalination process of zein glycopeptides. The results showed that zein glycopeptides was desalted by a 200 nanofiltration membrane was carried out continuously for 5 times nanofiltration under 2.0 MPa at 20 ℃ with each nanofiltration volumetric concentration factor of 2. After 5 times of nanofiltrations, the electrical conductivity of retentate was decreased by 60.87%. The desalting rate and the recovery rate of short zein glycopeptide were 81.60% and 96.97%, respectively. The hydroxyl radical scavenging rate of desalted zein glycopeptide was increased from 11.25% to 15.85%, and the 1,1-diphenyl-2-picrylhydrazyl radical scavenging rate and Fe²⁺-chelating activity were decreased by 11.83% and 46.07%, respectively. Moreover, the 200 nanofiltration membrane could remove 55.54% of unreacted D-glucosamine in the glycosylation reaction product, playing a triple role of desalination, desugarization and concentration.