LAMP在阪崎克罗诺杆菌检测中的研究进展

Progress of Loop-Mediated Isothermal Amplification in Detection of Cronobacter sakazakii

摘要:

阪崎克罗诺杆菌（Cronobacter sakazakii）是一类食源性致病菌，可引起新生儿脑膜炎、败血症等疾病。环介导等温扩增（Loop-Mediated Isothermal Amplification，LAMP）技术是一类新型的恒温核酸扩增技术，具有灵敏度高、耗时短、特异性强、对设备需求低等特点，可与多种检测方法结合应用于食源性致病微生物的检测。作者旨在阐明LAMP的基本原理并归纳结合不同检测方法的LAMP技术在阪崎克罗诺杆菌快速检测中的研究进展。

Abstract:

Cronobacter sakazakii is a kind of foodborne pathogenic bacteria, which can cause neonatal meningitis, septicemia and other diseases. Loop-mediated isothermal amplification technique (LAMP) is a new type of isothermal nucleic acid amplification technique with high sensitivity, short time-consuming, high specificity and low equipment requirements. It can be combined with a variety of detection methods for the detection of foodborne pathogenic microorganisms. This paper aims to elucidatethe basic principles of LAMP and summarize the progress of LAMP technology combined with different detection methods in the detection of C. sakazakii.