Abstract:Rapid and sensitive detection of various hazard factors in food is the key to ensure food safety and improve human health. Immunochromatographic test strip(ICTS) has become the most popular technique for rapid screening and on-site detection of food safety, exhibiting the advantages of low cost, simple operation, short assay time and no need of complex equipment and professional technicians. Particularly, the emergence of multiplex ICTS greatly improves the detection flux and shortens the overall analysis time, providing an effective technical support for simultaneous detection of various hazard factors in food. In this paper, five common signal output modes (colorimetry, fluorescence, and surface enhanced Raman scattering, chemiluminescence and magnetic signal) were introduced, the latest research progress on simultaneous detection of food hazard factors by integrating the above-mentioned signal outputs with multiplex ICTS platform was reviewed. The article aims to provide a certain theoretical guidance for the design and construction of multiplex ICTS technology and its application in food safety analysis.