根皮素对硫代乙酰胺诱导的小鼠急性肝损伤的保护作用

Hepatoprotective Effects of Phloretin on Thioacetamide-Induced Acute Liver Damage in Mice

摘要:

本实验旨在研究根皮素对硫代乙酰胺诱导的小鼠急性肝损伤的保护作用。采用硫代乙酰胺造模，诱导小鼠急性肝损伤，以槲皮素作为阳性对照药物，通过检测血清学指标（丙氨酸氨基转移酶ALT、天冬氨酸氨基转移酶AST、γ-谷氨酰转肽酶γ-GT、 碱性磷酸酶ALP和血清总胆红素TB）和组织病理切片方法，研究根皮素对小鼠急性肝损伤的保护作用。实验结果显示，在预先灌胃高、低剂量根皮素组别中，小鼠血清ALT 活性分别为（149.14±21.55） U/L和（189.43±16.64） U/L，AST 活性分别为（123.58±35.90） U/L和（168.64±33.32） U/L。γ-GT 活性分别为（103.19±20.93） U/L和（129.30±27.91） U/L，ALP 活性分别为（19.67±3.32） King U/dL和（24.17±3.58） King U/dL，血清TB浓度分别为（198.14±29.64） μmol/L 和（227.04±34.20） μmol/L。相比于造模组，根皮素组ALT、AST、γ-GT、ALP和TB明显降低（n-=8，p-<0.05），而且降低幅度呈现剂量依赖性，根皮素处理组的小鼠肝组织病理学病变得到了显著改善。研究表明，根皮素对硫代乙酰胺诱导的小鼠急性肝损伤有显著的保护作用，是值得进一步研究的急性肝损伤保护的候选药物和食品添加剂。

Abstract:

This study aimed to examine the hepatoprotective effects of phloretin on acute liver damage induced by thioacetamide(TAA) in Kunming mice. Quercetin was used as the positive control drug. The hepatoprotective effect was studied by means of serological index, including the serum levels of alanine aminotransferas(ALT), aspartate aminotransferase(AST), γ--glutamyl transferase(γ-GT), alkaline phosphatase(ALP), and total bilirubin(TB), as well as the histopathological changes. In the groups with high and low doses of phloretin, the serum ALT activity was (149.14±21.55) U/L and (189.43±16.64) U/L, respectively, and the serum AST activity was (123.58±35.90) U/L and (168.64±33.32) U/L, respectively. The serum γ-GT activity was (103.19±20.93) U/L and (129.30±27.91) U/L, respectively, and the ALP activity was (19.67±3.32) King U/dL and (24.17±3.58) King U/dL, respectively. The total serum bilirubin contents were (198.14±29.64) mol/L and (227.04±34.20) mol/L, respectively. Compared with the model group, phloretin significantly prevented the increase in serum ALT, AST, γ-GT, ALP and TB in acute liver damage induced by TAA(n-=8, p-<0.05), and the decrease was dose-dependent. A significant reduction was produced in the histopathological hepatic lesions. The results showed that phloretin had good hepatoprotective activity on acute liver injury induced by thioacetamide in mice, and it could be considered as a candidate drug and food additive worthy of further study.