利用LC-MS技术解析黑果枸杞、红果枸杞代谢物的差异

Analysis of the Differences of Metabolites between Lycium ruthenicum Murr. and Lycium barbarum L. by LC-MS

摘要:

为了分析比较黑果枸杞和红果枸杞代谢物的差异。利用液相色谱质谱联用技术（LC-MS）分析其代谢产物，共检测出6 982种代谢物，包括负离子2 704个，正离子4 278个；主成分分析（PCA）分析发现，黑果枸杞和红果枸杞可以被区分开；对差异代谢物的分析发现，二者共有501种差异代谢物，其中表达量前50的差异代谢物中有7种是黄酮类物质，6种是氨基酸和多肽类物质；11个代谢通路被显著富集，其中4个和氨基酸代谢相关。本研究为深入了解枸杞中的氨基酸代谢以及对黑果枸杞和红果枸杞的差异利用提供一定依据。

Abstract:

This study aimed to analyze and compare the differences of metabolites between Lycium ruthenicum< Murr. and Lycium barbarum L.. Liquid chromatography mass spectrometry technique (LC-MS) was adopted to analyze the metabolites. A total of 6 982 metabolites were detected, including 2 704 negative ions and 4 278 positive ions. Principle component analysis (PCA) displayed that Lycium ruthenicum Murr. and Lycium barbarum L. could be distinguished. Analysis of differential metabolites revealed a total of 501 differential metabolites. Among the top 50 differentially expressed metabolites, 7 were flavonoids, and 6 were amino acids and polypeptides. 11 metabolic pathways were significantly enriched, 4 of which were associated with amino acid metabolism. This study provided a basis for in-depth understanding of the biosynthesis of amino acid metabolism and the differential utilization of Lycium ruthenicum Murr. and Lycium barbarum L..