干贝多糖的蛋白质脱除方式对抗氧化活性的影响

Effect of Deproteinization Methods of Polysaccharide from Dried Adductor Muscle on Antioxidant Activities

摘要:

为比较蛋白质脱除方式对干贝多糖的组成、结构和抗氧化活性的影响，对热水辅助超声法提取的干贝柱多糖采用碱酶法、稀碱法、三氯乙酸（TCA）法、 D-葡萄糖酸-δ-内酯（GDL）法4种方式脱蛋白质，并比较脱蛋白质后4组多糖的多糖质量分数、单糖组成、氨基酸组成、三螺旋结构、微观结构及体外抗氧化活性。结果表明：TCA组多糖的质量分数最高，可达（74.83±0.57）%，4组多糖的单糖组成均以葡萄糖为主；GDL组总氨基酸质量分数、极性氨基酸质量分数与非极性氨基酸质量分数较高；碱酶组和GDL组多糖具有三螺旋结构；稀碱组多糖致密光滑，其余3组多糖疏松多孔。综合来看，碱酶组多糖的质量分数较高且抗氧化活性较好。不同的蛋白质脱除方式会影响干贝多糖的组成和抗氧化活性。

Abstract:

In order to compare the effects of deproteinization methods on the composition, structure and antioxidant activity of the dried adductor muscle polysaccharides, four deproteinization methods were applied to the polysaccharides extracted by hot water-assisted ultrasound method, including enzymatic hydrolysis, dilute alkali pretreatment, trichloroacetic acid(TCA) method and D-glucono-δ-lactone(GDL) method. The polysaccharide content, monosaccharide composition, amino acid composition, triple helix, microstructure and antioxidant activity in vitro of the four deproteinization groups were compared. The results showed that the polysaccharide content of TCA group was the highest, reaching （74.83±0.57）%. The monosaccharide composition of polysaccharides in the four groups was mainly glucose. The total amino acid content, polar amino acid content and non-polar amino acid content were higher in the GDL group. The polysaccharides of alkali-enzyme group and GDL group were in triple helix structure. The polysaccharides of dilute alkali group were dense and smooth, while polysaccharides of the other three groups were loose and porous. Comprehensively, the polysaccharide content of alkali enzyme group was higher and the antioxidant activity was better. Different deproteinization methods could affect the composition and antioxidant activity of polysaccharides extracted from adductor muscle.