芫荽的抗氧化活性及对高果糖高盐饮食诱导大鼠血压升高的缓解作用

Anti-Oxidative Activity of Coriander and Preventive Effects on Blood Pressure Elevation in Rats Induced by High-Fructose and High-Salt Diet

摘要:

通过测定清除DPPH自由基、羟自由基能力和还原能力，评估芫荽的体外抗氧化活性。通过大鼠实验研究芫荽拮抗血压升高的潜力。雄性SD大鼠被随机分为4组。正常组（NC）饲喂普通饲料和水，模型组（M）、芫荽低剂量（LP）和芫荽高剂量组（HP）均饲喂质量分数60%高果糖饲料和2 %盐水。实验开始1周后LP和HP组以1 g/（kg·d）和2 g/（kg·d）的芫荽冻干粉分别灌胃。固定时间测量大鼠血压。实验结束后，计算平均动脉压（MAP）并检测血清总胆固醇（TC）、甘油三酯（TG）、高密度脂蛋白（HDL-C）及低密度脂蛋白（LDL-C）含量。芫荽醇提物展现出良好的体外DPPH自由基清除能力、羟自由基清除能力和还原能力。体内实验结果表明，芫荽粉显著降低高糖高盐饮食诱发的大鼠体质量增加和血压升高。经高果糖高盐饮食喂养大鼠的平均动脉压（21.2 kPa）显著高于正常大鼠（15.8 kPa），而高剂量和低剂量芫荽粉分别干预6周后，大鼠平均动脉压分别下降至15.7、14.5 kPa，趋于正常水平。此外，芫荽干预有效拮抗高糖高盐饮食诱发的大鼠血清中TC、TG、HDL-C和LDL-C的异常改变。芫荽具有良好的体外抗氧化能力，并且可有效预防高果糖高盐饮食导致的大鼠血压升高。

Abstract:

The in vitro antioxidant capacity of coriander and its preventive effects on blood pressure elevation in rats induced by high-fructose and high-salt diet were investigated. The in vitro antioxidant activity of coriander was evaluated by measuring the ability to scavenge DPPH free radicals and hydroxyl free radicals, as well as the reducing ability. The preventive potential of coriander on blood pressure elevation induced by high-fructose and high-salt diet was assessed by conducting a rat experiment. Male SD rats were randomly divided into four groups. Rats in normal control group (NC) were fed with normal feed and water. Rats in model group(M), low-dose coriander group (LP) and high-dose coriander group (HP) were fed with high fructose (60%) feed and 2% saline. One week after the experiment, rats in LP and HP groups were daily administered with lyophilized coriander powder of 1 g/（kg·d） and 2 g/（kg·d）, respectively. The blood pressure of rats was measured at a fixed time every week. The mean arterial pressure(MAP) was calculated at the end of experiment, and the level of total cholesterol(TC), triglycerides(TG), high density lipoprotein(HDL-C) and low-density lipoprotein(LDL-C) in serum were also detected. The ethanolic extract of coriander showed excellent in vitroin vitro DPPH radical scavenging ability, hydroxyl radical scavenging ability and reducing ability. The results of in vivo experiment in rats showed that the lyophilized coriander powder effectively alleviated the increment of body weight and blood pressure induced by high-fructose and high-salt diet. The MAP of rats in M group (21.2 kPa) was significantly higher than that of the normal rats (15.8 kPa). After intervention for 6 weeks with lyophilized parsley powder under high-dose and low-dose, the MAP values remarkably decreased to the normal level, i.e., 15.7 and 14.5 kPa, respectively. Additionally, coriander effectively antagonized the abnormal alterations in serum TC, TG, HDL-C and LDL-C contents induced by high-fructose and high-salt diet. Coriander possesses excellent antioxidant capacity in vitro, and can effectively prevent the elevation of blood pressure in rats induced by high-fructose and high-salt diet.