茯砖茶对APOE-/-小鼠肝脂合成和氧化应激影响

Effect of Fu Brick Tea on Hepatic Lipid Synthesis and Oxidative Stress in APOE-/- Mice with Non-Alcoholic Fatty Liver

摘要:

观察茯砖茶对非酒精性脂肪肝治疗效果及对肝脂合成和氧化应激的影响。选取2月龄雄性APOE^-/-小鼠55只，连续高脂饲料喂养13周后随机抽取5只进行HE染色判断成模率。造模成功后随机分为模型组，阿托伐他汀组，茯砖茶高、中、低剂量组，持续高脂饲料喂养并灌胃干预8周。同时选2月龄C57小鼠作为空白对照组，普通饲料喂养13周后予以生理盐水连续灌胃8周。实验末取小鼠肝脏组织用于肝功能、氧化应激、HE染色及RT-qPCR。病理学显示茯砖茶高、中剂量组小鼠肝脂轻于模型组。与空白对照组比较，模型组肝组织GSH-PX、SOD活力较低；MDA质量摩尔浓度，ALT活力，AST活力，NAS积分，SCD-1、PPAR-γ、HMGCR基因表达较高（P<0.05）。与模型组比较，茯砖茶不同剂量组肝组织MDA质量摩尔浓度，ALT、AST活力，NAS积分，SCD-1、PPAR-γ、HMGCR基因表达较低；GSH-PX、SOD活力较高。茯砖茶通过抑制肝脂合成和减少肝脏氧化应激损伤而达到逆转早期非酒精性脂肪肝的效果。

Abstract:

The effect of Fu brick tea on hepatic lipid synthesis and oxidative stressin jury in APOE^-/- mice with non-alcoholic fatty liver was investigated in this study. Fifty-five male APOE^-/-mice aged 2 months were continuously fed with high fat diet for 13 week. Five of them were randomly selected for HE staining to estimate the successful development of animal model.The remaining fifty APOE-/- mice were randomly divided into model group,atorvastatin group,and high-, medium- and low-dose groups of Fu brick tea after successful modeling. All of APOE^-/- mice were respectively intervened with high fat diet through gavage for 8 weeks. At the same time, 2-month-old C57 mice 2 were selected as the control group and fed with normal diet for 13 weeks and then given saline for 8 weeks. At the end of the experiment, the liver tissues of mice were taken for liver function, oxidative stress, HE staining and RT-qPCR. Pathological morphology showed that the degree of liver steatosis in Fu brick tea of high- and medium-dose groups were lighter than that in the model group. Compared with the control group, the activity of GSH-PX and SOD in the liver of model group decreased, while the content of MDA, the activity of ALT and AST, NAS score, the gene expression of SCD-1、PPAR-γ and HMGCR increased (P<0.05). Compared with the model group, the content of MDA, the activity of ALT and AST, NAS score, the gene expression of SCD-1, PPAR-γ and HMGCR were decreased in different Fu brick tea dose groups, while the GSH-PX and SOD activity increased. Fu brick tea can reverse the early NAFLD by inhibiting hepatic lipid synthesis and reducing hepatic oxidative stress damage.