卵蛋白源胰脂肪酶抑制肽的制备及其对小鼠体质量控制效果

Preparation of Pancreatic Lipase-Inhibiting Peptides from Ovalbumin and Its Effect on Body Weight Control in Mice

摘要:

采用响应面法对卵蛋白源胰脂肪酶抑制肽（PLIPA）的制备工艺进行了研究，经模拟胃肠道消化研究PLIPA的稳定性。C57BL/6J小鼠饲喂高脂饲料40 d后成功建立肥胖模型，灌胃PLIPA 28 d后处死，检测相关指标，研究PLIPA对营养型肥胖小鼠体质量和体形的控制效果。结果表明，卵蛋白质量浓度80 g/L，加入2 240 U/g的碱性蛋白酶，在pH 9.0、55 ℃水解4 h，制备的PLIPA对胰脂肪酶的抑制率最高，为（56.06±2.64）%。经模拟胃液、胃液加肠液消化后，每毫克PLIPA对胰脂肪酶的抑制率分别为0.53%和0.46%，整体抑制率仍较高，稳定性较好。小鼠实验表明，PLIPA复合组的体质量和Lee′s指数显著低于肥胖模型组（P<0.05），处理组的腹部脂肪系数、甘油三酯（TG）、总胆固醇（TC）、低密度脂蛋白胆固醇（LDL-C）与高密度脂蛋白胆固醇（HDL-C）等指标均低于肥胖模型组，表明PLIPA对于肥胖小鼠具有减轻体质量和降脂作用，其中PLIPA复合组控制体质量和体形效果最好。

Abstract:

The preparation of pancreatic lipase-inhibiting peptide from ovalbumin(PLIPA) was studied by response surface method, and the stability of PLIPA was studied by simulated gastrointestinal digestion. C57BL/6J mice obesity model was successfully established after feeding high fat diet for 40 days. After 28 d of intragastric administration of PLIPA, the mice were killed and the related indexes were detected to study the control effect of PLIPA on body weight and body shape of nutritional obese mice. The results showed that when the concentration of ovalbumin and alkaline protease were 80 g/L and 2 240 U/g, respectively, the highest inhibitory rate of PLIPA was (56.06±2.64)% after hydrolyzed at 55 ℃, pH 9.0 for 4 h. After digestion of simulated gastric solution and simulated gastric and intestinal solution, the inhibitory rate of PLIPA per milligram of pancreatic lipase were 0.53% and 0.46%, respectively. The overall inhibition rate was still high and the stability was good. The body weight and Lee's index of PLIPA complex group were significantly lower than those of model group (P<0.05). The indexes of abdominal fat coefficient, triglycerides(TG), total cholesterol (TC), and low-density lipoprotein cholesterol(LDL-C) and high-density lipoprotein cholesterol(HDL-C) in the treatment groups were lower than those in model group, indicating that PLIPA could reduce body weight and fat in obese mice, and PLIPA complex group showed the best effect on controlling body weight and body shape.