玛咖复合黄精和桔梗调节糖脂代谢作用的研究

Effects of Maca, Polygonatum sibiricum and Platycodon grandiflorum on Glucose and Lipid Metabolism

摘要:

旨在探讨玛咖、黄精和桔梗3种原料的水提物复方，在细胞水平对调节糖脂代谢的作用。用超声波辅助法分别提取玛咖、黄精和桔梗的水溶性成分，得到玛咖（maca）水提物，将黄精（HJ）水提物和桔梗（JG）水提物按质量比为1∶1的比例混合得到黄精-桔梗（HJJG）水提物。采用游离脂肪酸、果糖和葡萄糖诱导人肝癌细胞株（HepG2）细胞，建立糖脂代谢紊乱模型。以二甲双胍作为阳性对照组，分别评价质量浓度为0.25、0.5、1 mg/mL的maca水提物、HJJG水提物以及两者复配物对模型细胞的影响。测定的指标包括模型细胞的葡萄糖吸收量、细胞内糖原、甘油三酯（TG）、总胆固醇（TC）的含量以及细胞内活性氧自由基（ROS）的水平。结果表明，玛咖和黄精-桔梗复配后能够通过促进糖吸收和细胞内糖原合成，以及降低细胞内TC含量和ROS水平P＜0.05），调节模型组HepG2细胞糖脂代谢，其中质量浓度为1 mg/mL的玛咖和1 mg/mL HJJG（1∶1，体积比）复配后改善效果较优。

Abstract:

This study was to investigate whether the combination of aqueous extracts from maca (Lepidium meyenii Walp)，Polygonatum sibiricum and Platycodon grandiflorum could effectively regulate glucose and lipid metabolism at cellular level. Ultrasonic-assisted water extraction was adopted to obtain their aqueous extracts. The HJJG aqueous extract was achieved by mixing the extracts form Polygonatum sibiricum (HJ) and Platycodon grandiflorum (JG) at a mass ratio of 1∶1. A glucose-lipid metabolic disorder model was established by inducing human hepatocellular carcinoma cell line (HepG2) with free fatty acid，fructose and glucose. Metformin was used as the positive control group to evaluate effects of aqueous extract of maca，HJJG or maca-JHHG mixture on the model cells at different concentration (0.25，0.5, 1 mg/mL). The glucose absorption，intracellular glycogen，triglyceride (TG)，total cholesterol (TC) and reactive oxygen radicals (ROS) levels in the model cells were measured. The results showed that glucose and lipid metabolism in model HepG2 cells could be significantly regulated by combing maca and HJJG aqueous extracts. They coordinately promoted the sugar absorption and intracellular glycogen synthesis. They also reduced the levels of TC and intracellular ROS in cells (P < 0.05)，especially. The mixture of maca (1 mg/mL) and HJJG (1 mg/mL) at a volume ratio of 1∶1 exhibited the best effect.