产κ -卡拉胶裂解酶菌株的筛选与鉴定

Screening, Isolation and Identification of κ -Carrageenan Lyase-Producing Bacteria

摘要:

利用单一碳源的方法，从13种来自辽宁省大连市不同坐标的海泥样品中，筛选得到4株具有κ-卡拉胶酶活性的菌株。利用DNS法（3，5-二硝基水杨酸法）检测4株菌具有κ -卡拉胶酶活性。根据酶活性比较进行复筛，得到一株高产κ -卡拉胶酶的菌株命名为LG-2，其最高酶活为34 U/mL，根据形态学观察、生理生化鉴定和16S rDNA系统发育树分析，初步确定菌株为红球菌属，为革兰氏阳性菌。并对LG-2号菌株生长曲线及产酶活性曲线进行检测，为工业化生产奠定基础。

Abstract:

Using κ -carrageenase as the sole carbon source, 4 strains with κ -carrageenase activity were screened from 13 samples of sea mud with different coordinates in Dalian, Liaoning Province. The DNS method (3,5-dinitrosalicylic acid method) was then used to detect the κ -carrageenase activity produced by 4 strains. According to the κ -carrageenase activity comparison, a high-yielding strain was named LG-2, with a maximum enzyme activity of 34 U/mL. Based on morphological observation, physiological and biochemical identification, and 16S rDNA phylogenetic tree analysis, the strain was initially identified as Rhodococcus and Gram-positive bacteria. The growth curve and enzyme activity curve of LG-2 strain were determined, laying the foundation for industrial production.