苍术挥发油杀菌活性评价及抑菌机制

Study on Bactericidal Activity and Antibacterial Mechanism of Volatile Oil of Atractylodes chinensis Koidz

摘要:

研究苍术挥发油体外杀菌活性及抑菌机制。以大肠埃希氏菌、金黄色葡萄球菌、白假丝酵母为供试菌，采用悬液定量杀菌实验测定苍术挥发油的杀菌活性，并测定对3种供试菌胞外碱性磷酸酶（AKP）、可溶性蛋白质含量和生长曲线的影响，从而阐述苍术挥发油的杀菌机制。结果表明，苍术挥发油对3种常见致病菌均有显著的抑制或杀灭作用。3种致病菌悬液中AKP、可溶性蛋白质等大分子物质含量比对照组显著增加，经苍术挥发油处理过的3种菌生长受到明显抑制。苍术挥发油能够通过改变菌体细胞膜通透性，破坏菌体完整结构发挥体外杀菌活性。

Abstract:

To evaluate the bactericidal activity in vitro and study the antibacterial mechanism of Atractylodes chinensis volatile oil. The antibacterial activity of Atractylodes chinensis volatile oil against Escherichia coli, Staphylococcus aureus and Candida albicans was evaluated by the suspension quantitative germicidal test. Its antibacterial mechanism was elucidated by measuring the effects on the leakage rate of extracellular alkaline phosphatase(AKP), soluble protein content and growth curve of the three strains. Volatile oil from Atractylodes chinensis could effectively inhibit or sterilize the tested microorganism. The contents of alkaline phosphatase(AKP) and soluble protein were significantly increased when compared with those in the control group, while the growth of indicators was suppressed significantly. The Atractylodes chinensis volatile oil showed prominent bactericidal activity by changing cell membrane permeability and destroying cell integrity.