富含鹅肌肽的鸡胸肉酶解物对小鼠降尿酸作用初探

Effect of Chicken Breast Hydrolysate Enriched with Anserine on Uric Acid in Mice

摘要:

探讨富含鹅肌肽的鸡胸肉酶解物对患有高尿酸血症小鼠的降尿酸作用。以不同来源的酶解物的不同剂量灌胃，观察氧嗪酸钾盐诱导的急性高尿酸血症小鼠脏器指数、血清中尿酸（Uric acid，UA）、肌酐（Creatinine，CRE）、尿素氮（Urea nitrogen，UN）浓度，检测肝脏黄嘌呤氧化酶（Xanthine oxidase，XOD）、腺苷脱氨酶（Adenosine deaminase，ADA）、谷草转氨酶（Aspartate transaminase，AST）、谷丙转氨酶（Alanine transaminase，ALT）的活性及甘油三酯（Triglyceride，TG）浓度。结果表明，富含鹅肌肽的鸡胸肉酶解物能有效缓解肝脏和肾脏指数，样品1和样品2的中剂量效果最为明显，且能有效降低血清的UA、UN和CRE水平，效果优于别嘌呤醇。样品1和样品2均能抑制小鼠肝脏ADA活力，效果与别嘌呤醇相差并不显著，但只有样品1的低剂量能显著降低XOD活力。样品1的鸡胸肉酶解物可以极显著降低ALT活性，且低剂量降低酶活性的效果强于别嘌呤醇，高剂量可极显著降低AST活性。酶解物对肝脏TG浓度的影响与对XOD的影响趋势具有一致性，证实了TG浓度与XOD活力具有相关性。富含鹅肌肽的鸡胸肉酶解物能有效降低尿酸水平，缓解小鼠的急性高尿酸血症。

Abstract:

The study aimed to investigate the uric acid lowering activity of chicken breast hydrolysate enriched with anserine in mice with hyperuricemia. Different sources of hydrolysates enriched with anserine were administrated with different doses to hyperuricemia mice induced by potassium oxonate. The effects of anserine on organ index, serum levels of uric acid (UA), creatinine (CRE) and urea nitrogenand (UN), activities of hepatic xanthine oxidase (XOD) , adenosine deaminase (ADA), aspartate transaminase (AST) and alanine transaminase (ALT), and the content of triglyceride were studied in hyperuricemia mice. Results showed that anserine could effectively alleviate the liver and kidney index. The medium dosage in sample 1 and sample 2 had the most obvious effect, and could effectively reduce the levels of UA, UN and CRE in serum, and the effect was better than allopurinol. Both sample 1 and 2 could inhibit the liver ADA activity in mice, and the effect was not significantly different from allopurinol. However, only the low dosage in sample 1 could significantly reduce XOD activity. Sample 1 could significantly reduce ALT activity, and low dose reduced enzyme activity more than allopurinol, high dose could significantly reduce AST activity. The effect of chicken breast hydrolysate on liver TG content was consistent with the trend of XOD, which confirmed the correlation between TG content and XOD activity. Therefore, chicken breast hydrolysate enriched with anserine can effectively reduce uric acid levels and alleviate hyperuricemia in mice.