香菇多糖纯化前后结构和生物活性的比较

Comparison of the Structural Characteristicsand Biological Activity of before and after Purification olysaccharides of L entinula edodes

摘要:

为了比较香菇多糖纯化前后的结构特性和生物活性。分别对香菇粗多糖样品（命名为L0）及其纯化后的3个多糖组分（依次命名L1、L2和L3）进行结构特性（紫外光谱，红外光谱，扫描电镜，粘均相对分子质量，刚果红络合物）和生物活性（抗氧化活性和抑菌活性）的测定。紫外光谱结果显示纯化后多糖组分基本不含有核酸和蛋白质；红外光谱结果表明多糖纯化前后均有显著的多糖特征吸收峰和β-糖苷键，其中L1与L0的结构更相似，吸收峰更明显；扫描电镜结果显示，纯化前后组分有一定的差异，其中L2和L3表面结构呈现出碎屑状堆积，而L1呈现出有一定凸起和褶皱的块状；刚果红络合物结果表明，L0和L1中含有三螺旋结构；L0、L1、L2和L3的粘均相对分子质量分别为2.19×105 、8.34×104 、4.40×104 、3.66×104。抗氧化实验表明，L0具有更好的抗氧化效果；对金黄色葡萄球菌抑菌圈的测定发现L1具有更好的抑菌效果。实验结果表明，纯化前后香菇多糖在结构和活性上均有一定的差异，为活性多糖组分的制备和利用提供了一定的依据。

Abstract:

The effect of purification on the structure and biological activity of polysaccharide in Lentinula edodes were investigated. The structural characteristics（UV，FT-IR，SEM，Viscosity- average molecular weight，Congo red complex）and biological activity（Antioxidant and Antibacterial activity）of crude polysaccharide samples （named L0）and their purified three polysaccharides （named L1，L2，and L3）were measured respectively. The UV results showed that nucleic acids and proteins were not found in the purified polysaccharides. The FT-IR results showd that all components have absorption peak of sugar and β-glucosidic bond，but the FT-IR structural of L1 were similar to L0. The SEM pictures showd that the surface structures of L2 and L3 were similar，presents the fragmental accumulation，and the structure of L1 present a bump and fold block. With Congo red complex of the study，L0 and L1 contained three spiral structure，the L2 and L3 did not contain three spiral structure. The antioxidation experiment showed that the L0 components had better anti-oxidation activity. The determination of staphylococcus aureus was found that L1 have better antibacterial activity. The work found that after purification，the structure and biological activity of polysaccharide were different. These provided some basis for the selection of active components.