蝙蝠蛾拟青霉治疗1型糖尿病活性研究

Study on the Anti-Diabetic Effects of Paecilomyces hepiali

摘要:

为探究蝙蝠蛾拟青霉菌丝体对1型糖尿病大鼠降血糖活性及机理，采用腹腔注射链脲佐霉素（STZ）方法建立1型糖尿病大鼠模型，系统考察蝙蝠蛾拟青霉菌丝体对糖尿病大鼠的降血糖、抗氧化及调节糖代谢方面的作用。通过检测大鼠血糖、体重发现，蝙蝠蛾拟青霉菌丝体灌胃4周后，显著提高大鼠体重，降低空腹血糖，具有极显著的降血糖作用。大鼠血清生化指标检测结果显示，蝙蝠蛾拟青霉菌丝体显著提高大鼠血浆胰岛素水平，促进葡萄糖吸收，加速糖代谢，抑制糖化血红蛋白合成；增加血清超氧化物歧化酶（Super-Oxide Dismutase，SOD）、谷胱甘肽过氧化物酶（Gluta-thione，GSH-Px）含量，提高机体抗氧化活性。蝙蝠蛾拟青霉菌丝体显著提高大鼠肝脏、肌肉糖原合成，抑制糖原降解。大鼠肾脏及胰岛组织病理切片显示，蝙蝠蛾拟青霉菌丝体能够有效改善糖尿病大鼠肾脏和胰岛组织病变，对糖尿病肾病具有很好的预防和治疗作用，同时能够减轻氧化应激对糖尿病大鼠胰岛形态结构的损伤。因此推断，蝙蝠蛾拟青霉菌丝体具有显著的降血糖活性，其活性可能是通过增加机体糖代谢及抗氧化活性实现的。

Abstract:

In type 1 diabetes mellitus rat model induced by STZ，the effects of Paecilomyces hepialid mycelium on hypoglycemic，antioxidation and glucose metabolism are investigated systemtically. Paecilomyces hepiali mycelium can effectively increase the levels of weight and reduce blood glucose in diabetes rat after 4-week administration. Biochemical indicators results show that Paecilomyces hepiali mycelium significantly enhanced the contents of insulin，promoted glucose absorption，accelerated glucose metabolism and inhibited glycosylated hemoglobin synthesis in serum. Paecilomyces hepiali mycelium improvs antioxidant activity through enhancing the levels of superoxide dismutase（SOD） and glutathione peroxidase（GSH-Px）. Furthermore，Paecilomyces hepiali mycelium increases the glycogen synthesis of liver and muscle and inhibited the degradation of glycogen. Pathological sections of kidney and pancreas in rats show that Paecilomyces hepiali mycelium improves the pathological changes of diabetic nephropathy indicating its therapeutic effect. Collectively，Paecilomyces hepiali mycelium has significant hypoglycemic activity，and its activity may be achieved by increasing the body's glucose metabolism and antioxidant activity.