胶质芽孢杆菌SM-01胞外多糖提取纯化工艺

Isolation and Purification of Exopolysaccharide from Bacillus mucilaginosus SM-01

摘要:

针对胶质芽孢杆菌SM-01发酵液粘度高，菌液分离困难而导致胞外多糖提取效率低的问题，采用硅藻土吸附-抽滤法，对提取工艺及超滤条件进行了研究，确定了工艺参数。结果表明，发酵液中NaCl添加量为3 g/dL，发酵液稀释倍数为3倍，硅藻土添加量为10 g/L时，在室温下抽滤可除去菌体和蛋白质。滤液在0.1 MPa下经截留相对分子质量为200 000的超滤膜超滤纯化，多糖回收率可达73.2%。

Abstract:

Since the broth of Bacillus mucilaginosus SM-01 was of high viscosity and hard to be separated，the diatomite sorption-filtration method was applied to improve the extraction efficiency of exopolysaccharide by optimizing the process and ultrafiltration conditions. The bacteria and protein of the broth can be removed facilely by filtration at room temperature under the optimal conditions included：the addition of NaCl is 3 g/dL，the dilution ratio of the broth is 3 times，and the amount of diatomite is 10 g/L. The filtrate can be purified by ultrafiltration（relative molecular-weight cut-off of 200 000） at 0.1 MPa. The recovery of exopolysaccharide was up to 73.2%.