滇结香花提取物组分1（Fr.1）对C2C12肌细胞胰岛素抵抗的改善作用

Ameliorative Effects of Fraction 1 from the Flower of Edgeworthia gardneri（Wall.） Meisn on Insulin Resistance of C2C12 Cells

摘要:

探讨滇结香花正己烷提取物组分1（Fr.1）改善棕榈酸（Palmitic acid，PA）诱导C2C12肌细胞产生胰岛素抵抗（Insulin Resistance，IR）的效果及其作用机制。方法：本文采用PA诱导C2C12肌细胞建立稳定的胰岛素抵抗细胞模型，通过检测Fr.1对胰岛素抵抗细胞（C2C12/IR）葡萄糖消耗量、葡萄糖摄取量的变化，研究其对PA介导的AMP活化蛋白激酶（AMPK）、胞内磷脂酰肌醇激酶/丝氨酸苏氨酸蛋白激酶（PI3K/AKT）信号通路中相关基因及蛋白表达的影响。结果：0.75 mmol/L PA作用C2C12肌细胞16 h作为最佳建模条件；Fr.1在无细胞毒性范围内（12.5~100 μg/mL）可显著增加C2C12/IR对葡萄糖的消耗量及摄取量；Fr.1可明显上调胰岛素受体（Insulin receptors，IRs）、胰岛素受体底物（Insulin receptor substrate-1，IRS-1）、葡萄糖转运蛋白4（Glut4）、PI3K、AMPKα基因的表达水平，并上调p-IRs、p-IRS-1、Glut4、p-AMPKα、磷酸化乙酰辅酶A羧化酶（p-ACC）、p-AKT蛋白质的表达水平，表明滇结香花提取物Fr.1可通过调控多个信号通路改善IR。

Abstract:

To investigate the ameliorative effects of n-Hexane extract Fraction 1（Fr.1） from the flower of Edgeworthia gardneri（Wall. ） Meisn on insulin resistance（IR），we established IR cell model of C2C12 by using palmitic acid（PA）. Glucose consumption，glucose uptake，relative mRNA and protein expression were detected by the glucose oxidase method，2-NBDG uptake，qRT-PCR and western blot analyses. 0.75 mmol/L PA and 16 hours were the optimal concentration and time to induce IR in C2C12 cells. In the range of non-cytotoxic concentration（12.5~100 μg/mL），Fr.1 promoted glucose consumption and uptake in C2C12/IR. Fr.1 also significantly upregulated Insulin receptors（IRs），Insulin receptor substrate-1（IRS-1），Glut4，PI3K and AMPKα mRNA expression and increased p-IRs，p-IRS，Glut4，p-AMPKα，p-ACC and p-AKT protein expression. Fr.1 from the flower of E. gardneri ameliorated IR through modulating IRs/IRS-1/PI3K/Akt/Glut4 and AMPK/ACC signaling pathways in C2C12 cells.