紫阳富硒茶对人肝癌细胞凋亡过程的诱导

Ziyang Se-Rich Tea Induced Apoptosis in Human Hepatoma Cells

摘要:

通过运用倒置荧光显微镜和流式细胞仪观察和测定细胞形态、周期、和凋亡率的变化，从而分析紫阳富硒茶对人肝癌细胞增殖的抑制作用及其诱导凋亡途径。结果表明：紫阳富硒茶能抑制人肝癌细胞的生长，并诱导人肝癌细胞凋亡；同时随着紫阳富硒茶质量分数的增加，人肝癌细胞形态发生明显变化，细胞周期被阻滞在S期，细胞凋亡率显著增加。试验表明紫阳富硒茶能抑制人肝癌细胞增殖，通过阻滞细胞周期从而诱导细胞凋亡。

Abstract:

This study aims to investigate the influence of Ziyang se-rich tea on the proliferation and apoptosis of HepG2 cells（a kind of human hepatoma cells）. The inverted fluorescence microscope and the flow cytometer are used to observe and test the changes of the cellular morphology，cell cycle and apoptosis. The results show that Ziyang se-rich tea could inhibit the growth of HepG2 cells. The cell morphology changes significantly. The cells cycle of HepG2 are arrested at the S-phase and the amount of the apoptotic cells increases obviously. It is concluded that Ziyang se-rich tea could inhibit the growth of HepG2 cells by blocking the cell cycle and induce the mitochondrial apoptotic pathway in a dose-dependent manner.