固态发酵废菌棒高产哈茨木霉孢子

Solid-State Fermentation of Trichoderma harzianum for High Yield Spore with Waste Bacteria Stick

摘要:

以废菌棒为固态发酵主要基质，采用单因素和响应面分析法（RSM）优化哈茨木霉固态发酵生产孢子，最后进行固态发酵放大实验。结果显示：最佳培养条件为接种量（质量浓度）7.5 g/dL，装料量20 g，pH自然，培养温度28 ℃，发酵周期144 h；响应面分析法优化的理论值为：初始含水质量分数57.07%，淀粉质量分数3.08%，NaNO3质量分数1.99%，理论孢子产量5.65×109 个/g，验证实验结果5.91×109个/g，符合理论预测；使用10 L自制固态发酵罐进行放大实验，通风条件下孢子产量显著提高，达到8.20×109个/g。

Abstract:

The solid-state fermentation of Trichoderma harzianum，in which waste bacteria stick were used as substrate，was optimized by using single-factor test and response surface methodology（RSM）. The up-scaled solid-state fermentation was carried out. Results showed that the optimal conditions were：inoculation 7.5 g/dL（w/w），substrate loading quantity 20 g，natural pH，culture temperature 28 ℃ and fermentation time 144 h. The optimal conditions of response surface analysis method were 57.07%（w/w） initial water content，3.08% starch and 1.99% NaNO3. The theoretical spore yield was 5.65×109 cfu/g compared with the actual yield 5.91×109 cfu/g，which was consistent with the theoretical prediction. The eventual sporulation was 8.20×109 cfu/g at the condition of ventilation by using the 10 L solid-state fermenter.