工业大麻雌株花叶多糖抑菌活性及稳定性分析

Antibacterial Activities and Stabilities of Polysaccharide from Flowers and Leaves of Female Plant in Industrial Hemp

摘要:

通过水提醇沉法制备工业大麻雌株花叶多糖（polysaccharide from flowers and leaves of female plant in industrial hemp，PFLFIH），采用Kirby Bauer纸片扩散法（K-B纸片琼脂扩散法）测定该多糖对9种常见致病菌的体外抑菌活性，同时研究了紫外线、温度、pH值、氧化剂与还原剂对其抑菌效果的影响。结果表明：PFLFIH对金黄色葡萄球菌具有杀菌效果，最低抑菌浓度（minimum inhibition concentration，MIC）为3.125 mg/mL，最低杀菌浓度（minimum bactericidal concentration，MBC）为6.25 mg/mL，对大肠埃希菌等其他8种人体致病菌无抑菌活性。该多糖具有良好的紫外稳定性和热稳定性，对氧化剂和还原剂的耐受能力较好；pH值对其抑制金黄色葡萄球菌活性的影响较大，在pH值为7时抑菌活性最高。

Abstract:

Polysaccharide from flowers and leaves of female plant in industrial hemp（PFLFIH） was extracted by decoction and alcohol sedimentation technique. Its antimicrobial activities in vitro of 9 kinds of normal pathogenic bacteria and fungi were determined by comparing the size of K-B inhibition zone. The effects of ultraviolet rays，temperature，solvents pH value，oxidant and reducer on its antimicrobial activities were also discussed. The results showed that PFLFIH had a good bactericidal effect on Staphylococcus aureas. Its minimum inhibitory concentration（MIC） was 3.125 mg/mL and minimum bactericidal concentration（MBC） was 6.25 mg/mL. Meanwhile，it has no antimicrobial activities of other 8 pathogenic bacteria and fungi. High temperature，ultraviolet rays，oxidant and reducer treatments did not affect its inhibitory effect. While pH value of the solvent environment is 7，the best antimicrobial activity could be shown.