红缘拟层孔菌多糖分离纯化、结构表征及其免疫活性分析

Isolation，Purification，Structural Characterization and Immunological Activity of Fomitopsis pinicola Polysaccharides

摘要:

红缘拟层孔菌是一种药用蘑菇以及有待开发利用的食品新资源。从红缘拟层孔菌中提取出水提多糖（FPS）和碱提多糖（FPJ），用DEAE Sepharose Fast Flow和Sepharose CL-6B对FPS进行分离纯化，首次得到4个多糖级分FPS1-1、FPS1-2、FPS2-1、FPS2-2。MTT实验表明，4个多糖级分均具有一定的免疫活性，其中FPS1-2和FPS2-1具有一定的量效关系，FPS2-1的免疫活性高于其他3个级分。HPSEC法测得FPS1-2、FPS2-1两种级分均呈单一峰，重均相对分子质量分别为9.10×103和3.02×105。经单糖组成、红外色谱和核磁共振分析，FPS1-2为含有α-（1→6）糖苷键链接方式的杂多糖，FPS2-1为含有α-（1→4）糖苷键链接方式的杂多糖。

Abstract:

Fomitopsis pinicola is a medicinal mushroom and a kind of new resource of food to explore. Two water-soluble crude polysaccharides were extracted using hot water and 5% of NaoH solution.，and then purified FPS by DEAE-Sepharose FF and Sepharose Cl-6B chromatography. Four polysaccharide factions named as FPS1-1，FPS1-2，FPS2-1 and FPS2-2 were isolated and purified from FPS. The result of the MTT test about normal mice spleen lymphocyte proliferation promotion activity indicated that the four polysaccharide factions both have a immunological activity. The effect of FPS1-2 and FPS2-1 are in a dose-dependent manner，and the effect of FPS2-1 was better than others. The HPSEC analysis indicated that the two fractions were all homogeneous polysaccharides and their relative molecular mass were respectively 9.10×103 and 3.02×105. The analysis of monosaccharide composition，infrared spectrum and NMR spectroscopy indicated that FPS1-2 was a heteropolysaccharide，it have α-（1→6）glucosidic bond；and FPS2-1 was a heteropolysaccharide ，it have α-（1→4）glucosidic bond.