氮源对 Nannochloropsis oculata生化组成、超微结构及生产的影响

Effects of Nitrogen Source on Biochemical Composition，Cell Ultrastructure and Production of Nannocloropsis oculata

摘要:

为了提高眼点微拟球藻生物量及生产强度，比较了氯化铵、乙酸铵及硝酸钠三种氮源对其生理生化及生产的影响。结果显示：铵盐组胞内蛋白质质量分数高于硝酸钠组，叶绿素a质量分数结果相反； 乙酸铵组总油质量分数最高，为13.5%； 硝酸钠组总碳水化合物质量分数最高，为16.5%。硝酸钠组极性油/中性油比值及EPA相对含量最高，分别为5.9和26.6%。铵盐组藻细胞尺寸大于硝酸钠组；硝酸钠组叶绿体数量、相对体积、类囊体密度高于铵盐组； 仅乙酸铵组胞内含有油体。乙酸铵组生物量及生产强度最大，分别为873 mg/L和25.3 mg/（L·d）。

Abstract:

In order to increase the biomass and productivity of Nannochloropsis oculata，the effects of three kinds of nitrogen sources (ammonium chloride，ammonium acetate and sodium nitrate) on physiology-biochemistry and production of N.oculata were compared in this research paper. The total cellular protein content of ammonium groups (ammonium chloride and ammonium acetate) were higher than that of sodium nitrate group，while cellular chlorophyll a content showed the opposite result； the total cellular oil content of ammonium acetate group was the highest，being 13.5%； the total cellular carbohydrate content of nitrate sodium group was the highest，being 16.5%. The polar lipid to neutral lipid ratio and the relative content of EPA of sodium nitrate group were the highest，being 5.9 and 26.6% respectively. The microalgal cell size of the ammonium groups were larger than that of sodium nitrate group； the number，relative volume and thylakoid density of chloroplast of sodium nitrate group were higher than those of ammonium groups； only the cells of ammonium acetate group contained oil bodies. The results showed that the biomass and productivity of ammonium acetate group were the highest，being 873 mg/L and 25.3 mg/(L·d) respectively.