青钱柳活性成分对IR-HepG2细胞葡萄糖消耗量及α-葡萄糖苷酶活性的影响

Effects of Cyclocarya paliurus（Batal.） Iljinskaja Extracts on Glucose Consumption of Insulin Resistance HepG2 Cell and Activity of

摘要:

研究青钱柳多糖（CPP）和青钱柳黄酮（CPF）对胰岛素抵抗的人源肝癌细胞（IR-HepG2）葡萄糖消耗量及α-葡萄糖苷酶活性影响。用高浓度胰岛素诱导培养HepG2细胞，建立IR-HepG2细胞模型，将IR-HepG2细胞分为模型组、CPP高、中、低剂量组、CPF高、中、低剂量组和二甲双胍组，各组以相应药物孵育24 h，采用葡萄糖试剂盒测定细胞葡萄糖消耗量（ΔGC），MTT法测定单位细胞葡萄糖消耗量（ΔGC/OD）；以阿卡波糖为阳性对照，比较不同浓度梯度的CPP与CPF对α-葡萄糖苷酶活性影响。与模型组比较，阴性对照组、二甲双胍组、CPP组与CPF组细胞?驻GC与ΔGC/OD显著性升高；不同浓度的CPP与CPF对α-葡萄糖苷酶均有抑制作用。提示青钱柳活性提取物降血糖功效与其提高细胞葡萄糖摄取量，抑制α-葡萄糖苷酶活性有关。

Abstract:

The effects of Cyclocarya paliurus（Batal.） Iljinskaja Extracts on glucose consumption of insulin resistance HepG2 cell and activity of alpha-glucosidase is studied. HepG2 cell with high concentration insulin（10 μg/mL） is induced to establish insulin resistance cell model and divided model cell into Cyclocarya paliurus polysaccharide（CPP），Cyclocarya paliurus flavone（CPF） and dimethylbiguanide groups. After 24 h administration，glucose assay is used fit to measure the gross glucose consumption of cells（ΔGC）， glucose consumption of unite cell（ΔGC/OD） is detected by MTT assay. The inhibitory activity on alpha-glucosidase of CPP and CPF is evaluated，compared with acarbose，in vitro. Compared with model group，ΔGC and ?驻GC/OD of IR-HepG2 cell is increased by CPF and CPP significantly. CPP and CPF have inhibitory effect on the activity of alpha-glucosidase. Those results indicat that CPF and CPP have effects on increasing the glucose consumption of cell and decreasing the activity of alpha-glucosidase，thus alleviating hyperglycemia.