纤维二糖2-差向异构酶的酶学性质及在乳制品中的应用

Enzymatic Properties of Cellobiose 2-Epimerase and Its Application in Dairy Products

摘要:

近年来，对纤维二糖2-差向异构酶（CE）的研究取得了许多突破性的进展。针对不同的寡糖，CE表现出不同程度的差向异构化和异构化活性，催化乳糖生成的依匹乳糖和乳果糖，两种都是有益生元性质的乳糖衍生物，在保健食品及药品中具有非常广阔的前景。因此，CE在工业应用的中游和下游工艺越来越引起重视，来自嗜温和嗜热微生物的CE也被应用于不同需求的生产中。尤其是嗜温微生物来源的CE能在较低温中保持活性，推进了CE在乳制品行业中的应用。作者针对性总结了CE的酶学性质和催化产物的最新研究进展，及其在乳制品行业中的发展。

Abstract:

In recent years，many substantial advances have been made in the research of cellobiose 2-epimerase（CE）. For different oligosaccharides，CE shows different levels of epimerization and isomerization activities，converting lactose into epilactose and lactulose，respectively. Both of them are lactose derivatives with prebiotic properties and show wide application prospects in food and pharmaceutical industries. Therefore，more and more attention has been attracted to the middle and downstream processes of industrial applications of CE. CEs from mesophilic and thermophilic microorganisms have also been applied to satisfy different needs in production. In particular，CE of mesophilic microorganisms can maintain activity at lower temperatures，facilitating the applications of CE in dairy industry. In this paper，we summarized the recent progress in the enzymatic properties，catalytic products，and dairy industrial development of CE.