臭氧降解玉米赤霉烯酮及其降解产物细胞毒性

Effects of Ozone on Zearalenone Degradation and Cytotoxicity Assessment of Ozonolytic Products

摘要:

臭氧处理玉米赤霉烯酮标准溶液后，产生了4种降解产物，其质荷比分别是335.184 1、351.190 7、321.186 8和367.175 3。应用CCK-8法研究细胞生长抑制率发现，玉米赤霉烯酮经臭氧处理后，肝癌细胞的增殖抑制率明显下降，但玉米赤霉烯酮降解产物对肝癌细胞生长仍存在抑制。作者通过超高效液相色谱串联质谱建立了一种在臭氧处理玉米赤霉烯酮过程中，同时检测玉米赤霉烯酮及其两种主要降解产物的方法。该方法在臭氧处理玉米赤霉烯酮污染的玉米粉中，能够检测出与臭氧处理玉米赤霉烯酮标准溶液时相同的两种主要降解产物，并在90 min臭氧处理后，玉米赤霉烯酮质量分数减少95.1%。

Abstract:

An ozonolytic study of zearalenone in acetonitrile solution was conducted under ozone treatment，producing four intermediate compound（m/z for Compound 1=335.184 1，Compound 2 = 351.190 7，Compound 3=321.186 8 and Compound 4=367.175 3）. By means of CCK-8 kit，cytotoxicity assessment of the degraded zearalenone products in HepG2 cells indicates continued toxicity，albeit at decreased levels.AUPLC-MS/MS method has been established to detect the two main products of zearalenoneozonolysis. Both products occur in practical process model for detoxifying contaminated corn flour by ozonation and zearalenone was decomposed by 95.1% in 90 minutes.