D-阿洛酮糖对Wistar大鼠脂质代谢作用的研究

D-Psicose，Favorably Alters Lipid Metabolism in Wistar Rats

摘要:

作者对D-阿洛酮糖改善Wistar大鼠的体质量，血脂水平，以及脂质代谢的可能机制进行了研究。将4周龄的Wistar大鼠随机分成5组：基础对照组（AIN-76A）、葡萄糖组（AIN+Glu）、果糖组（AIN+Fru）、阿洛酮糖组（AIN+Psi）以及纤维素组（AIN+Cel）。喂养4周后处死老鼠，分别测定血清中总胆固醇（TC）、甘油三脂（TG）、低密度脂蛋白胆固醇（LDL-C）以及游离脂肪酸（FFA），显微镜观察肝脏组织切片，同时对肝脏中的PPAR-α、FAS基因的表达进行量化实验。结果显示：在这5组中，D-阿洛酮糖组大鼠的体重变化量最小，血清中TG、游离脂肪酸、低密度脂蛋白含量明显下降（p<0.05），但TC变化不显著。RT-PCR结果表明，AIN+Psi组肝脏的PPAR-α表达显著上升（p<0.05），FAS表达显著下降（p<0.05），另外AIN+Glu、AIN+Fru及AIN+Cel组的FAS基因表达都较控制组高。这些结果表明，与传统糖类相比，D-阿洛酮糖饮食可以减少脂肪生成，控制血脂水平，对于体重控制具有很大的潜力。

Abstract:

This paper was designed to investigate the D-Psicose improved Wistar rat body weight，blood lipid levels，as well as the possible mechanisms of lipid metabolism. The rats were randomly divided into five groups：control group（AIN-76A），glucose group（AIN+Glu），fructose group（AIN+Fru）、D-psicose group（AIN+Psi） and cellulose group（AIN+Cel）. The rats were sacrificed after four weeks and the concentration of total cholesterol（TC），triglyceride（TG），low density lipoprotein cholesterol（LDL-C） and free fatty acid（FFA） in serum were measured. Liver histological analysis was performed by microscopy. The mRNA expression of PPAR-α and FAS in liver were measured by RT-PCR. The result showed that compared with the other diet groups，the body weight，serum TG，FFA，LDL-C level of AIN+Psi group were significantly decreased（P<0.05），but the TC was not significant. RT-PCR result showed that the expression of PPAR-α was up-regulated and FAS were significantly down-regulated in AIN+Psi group（P<0.05），in addition the expression of FAS in AIN+Glu，AIN+Fru and AIN+Cel were higher than AIN-76A. In summary，these results indicate that the D-psicose diet decreases lipogenesis，control blood lipid levels，leading to D-psicose's potential for weight management.