血红密孔菌Pycnoporus sanguineus SYBC-L10产纤维二糖脱氢酶的发酵优化

Culture Optimization for Cellobiose Dehydrogenase Productionby Pycnoporus sanguineus SYBC-L10

摘要:

研究了血红密孔菌Pycnoporus sanguineus SYBC-L10产纤维二糖脱氢酶的发酵优化。该酶是诱导酶，微晶纤维素是其最好的诱导底物。通过对发酵培养基中培养成分的优化，添加大分子碳源微晶纤维素和小分子碳源葡萄糖作为复合碳源，发酵优化后提高到78±5.94 U/L，是未优化前的2倍（39±4.78 U/L）。通过对氮源的优化，即有机氮源黄豆粉和无机氮源磷酸氢二铵的添加，使酶活达到700±35.78 U/L，比优化前提高了近17倍。而通过四因素四水平的碳氮源正交实验之后酶活达到1245±35.78 U/L，比优化前提高了近32倍。由此，确定了当微晶纤维素为7 g/L，葡萄糖5 g/L，黄豆粉6 g/L，磷酸氢二铵2 g/L时，其他不变，此时的产酶最高，达到1 245±35.78 U/L。

Abstract:

Effects of various parameters on cellobiose dehydrogenase（CDH） production by Pycnoporus sanguineus SYBC-L10. CDH was induced by cellulosic，and microcrystalline cellulose with high crystallinity supported the maximum CDH production. Though the optimization of fermentation medium composition，microcrystalline cellulose and glucose as the complex carbon source were add to the culture，the production of CDH reached 78±5.94 U/L，with 2 folds higher than that before optimization（39±4.78 U/L）.Though adding the soybean meal and（NH4）2HPO4 to the culture，the production of CDH reached 702±25.68 U/L，with 17 folds higher than that before optimization. Selecting the optimum carbon sources and nitrogen sources to get the optimum liquid medium recipe by the orthogonal test in four factors and four levers，The production of CDH reached 1245±35.78 U/L. The optimum fermentation medium consisted：microcrystalline cellulose 7 g/L，glucose 5 g/L，soybean meal 6 g/L，（NH4）2HPO4 2 g/L. The production of CDH reached 1 245±35.78 U/L.