乌鸡低聚肽铁的分离纯化及结构鉴定

Separation，Purification and Structural Identification of Iron-Chelating Black-Bone Silky Fowl Oligopeptides

摘要:

为了考察乌鸡低聚肽铁中肽段的结构，以乌鸡为原料，以氯化亚铁为铁源制备乌鸡低聚肽铁，对其总蛋白质质量分数、螯合率、得率进行测定，结果表明乌鸡低聚肽铁总蛋白质质量分数为（56.79±0.18）%，螯合率为（83.53±0.15）%，得率为（40.86±0.14）%。然后利用反相高效液相色谱（RP-HPLC）对其进行分离纯化，收集4个组分峰，利用四极杆飞行时间串联质谱仪（Q-TOF质谱仪）测定肽序列，分析出1个肽段，氨基酸序列为Thr-Ser-Gly-Met-Pro，相对分子质量为491.56。

Abstract:

In order to investigate the structure of peptides fromiron-chelating black-bone silky fowloligopeptides （BSFP-Fe），BSFP-Fe wasprepared by using black-bone silky fowl as raw material andiron chloride as iron resource. The total protein content，chelating rate and yield were determined. Results showed that the total protein content，chelating rate and yield were 56.79±0.18%，83.53±0.15% and 40.86±0.14%，respectively. Then，BSFP-Fe was separated and purified by reversed phase high performance liquid chromatography（RP-HPLC）.A total of4 fractions were collected and subjected to Q-TOF mass spectrometer to identify peptide sequences. One peptide，Thr-Ser-Gly-Met- Pro，was identified and its molecular weight was 491.56 u. This studyconfirmed the structure of peptides from BSFP-Feand provided certain theoretical support for the development of BSFP-Fe as iron supplement food.