β-紫罗兰酮脂质体包封率测定方法研究

Determination of Entrapment Efficiency of Beta-Ionone Liposomes

摘要:

采用薄膜超声法制备β-紫罗兰酮脂质体，通过反透析法分离脂质体和游离物质，并以高效液相色谱法为分析手段对其包封率进行测定。在选定色谱条件下，辅料不干扰测定，β-紫罗兰酮在5~80 mg/L的质量浓度范围内线性关系良好（R2=0.999 8），日内和日间精密度（RSD）均小于3%；透析平衡时间为12 h，游离物回收率符合要求（99.21%~101.22%），且透析20 h内无渗漏，方法重现性好；使用此法测得β-紫罗兰酮脂质体的平均包封率为（52.76±1.10）%。反透析法简便，准确，经济，适用于β-紫罗兰酮脂质体包封率的测定。

Abstract:

A method used for determining the entrapment efficiency of beta-ionone in liposomes was established. The liposome was prepared by thin film evaporation. The free beta-ionone was separated from liposomes by reverse dialysis and quantified with HPLC. The results showed that the calibrated curve was linear（R2=0.999 8） within the range of 5~80 mg/L for beta-ionone. Intra-day and inter-day precision（RSD）were both less than 3%. The equilibrium time for the dialysis was 12 h， and the recoveries showed with good reproducibility（99.21%~101.22%），and the leaking percentage in 20 h was negligible. The average entrapment efficiency of beta-ionone liposome was 52.76±1.10%. The reverse dialysis method was convenient，accurate and economic，suitable for determining the encapsulation efficiency of beta-ionone liposomes.