高效液相色谱分析虫草中核苷类成分

Determination of Nucleosides in Caterpillar Fungi by High Performance Liquid Chromatography

摘要:

为虫草中核苷类成分研究和质量控制提供参考，建立了一种高效液相色谱（HPLC）定量分析胞苷酸、尿苷酸、尿嘧啶、鸟苷酸、肌苷酸、次黄嘌呤、尿苷、腺苷酸、胸腺嘧啶、腺嘌呤、 2′-脱氧腺苷酸、肌苷、鸟苷、2′-脱氧鸟苷、胸苷、腺苷、2′-脱氧腺苷、虫草素和N6-（2-羟乙基）腺苷这19种核苷的方法。采用X select HSS T3 column（5 ?滋m，4.6 mm×250 mm）色谱柱，流动相为乙腈和水，梯度洗脱，流速1 mL/min，柱温40 ℃，检测波长254 nm。研究表明：19种核苷精密度RSD为0.11%～2.82%，稳定性RSD为0.22%～1.63%，保留时间RSD为0.14%～3.47%，重复性RSD为1.3%～5.2%，回收率为84.9%~108.3%，说明该方法准确、可靠，采用该方法成功测定了多种虫草产品中核苷的质量分数。

Abstract:

In order to afford a reference for the study of nucleosides and quality control of caterpillar fungi products，a method for simultaneous determination 19 kinds of nucleosides using HPLC was established including CMP，UMP，uracil，GMP，IMP，hypoxanthine，uridine，AMP，thymine，adenine， dAMP，inosine，guanosine，2'-deoxyguanosine，thymidine，adenosine，2'-deoxyadenosine，cordycepin， N6-（2-hydroxyethyl）-adenosine. Chromatographic conditions：X select HSS T3 column（5 ?滋m，4.6 mm×250 mm），acetonitrile and water as mobile phase with gradient elution，flow rate 1 mL/min，column temperature 40 ℃，detection wavelength 254 nm. Methodological research showed this was an accurate and reliable method which RSD of precision ranged 0.11%~2.82%，RSD of stability ranged 0.22%~1.63%，RSD of retention time ranged 0.14%~3.47%，RSD of reproducibility ranged 1.3%~5.2%，and recovery ranged 84.9%~108.3%. The nucleoside content in some caterpillar fungi products were successfully determined using this method.