光滑球拟酵母转录因子Asg1p调控鲁棒性的生理机制

Physiological Mechanism of Transcription Factor Asg1p for the Regulation of Robustness of Candida glabrata

摘要:

为了阐明缺失转录因子CgAsg1p对光滑球拟酵母鲁棒性的影响，通过同源重组的方法构建突变菌株Cgasg1?驻，比较基因缺失突变株在不同条件下的细胞生长、基因转录水平和对不同胁迫的抵御能力。结果表明：与野生型菌株wt相比，突变菌株Cgasg1?驻失去在pH 2.0下的生长能力，转录因子CgAsg1p在低pH条件下通过改变一系列应答基因的转录水平，影响压力调控、氧化还原过程、跨膜转运、DNA复制、重组和修复等生物过程，此外，突变菌株Cgasg1?驻也失去了对氧化胁迫和高渗胁迫的抵御能力。因此，CgAsg1p是光滑球拟酵母抵御多种胁迫的关键转录因子。

Abstract:

To analyze the effect of transcription factor CgAsg1p on the robustness of Candida glabrata，the mutant strain Cgasg1?驻 was constructed and the cell growth，gene transcriptional level and the ability of resisting various stresses were investigated. Compared to the wild-type strain C. glabrata ATCC 2001，the mutant strain Cgasg1?驻 lost the ability of surviving in the medium at pH 2.0. CgAsg1p changed the transcription level of several response genes，influencing stress regulation，redox process，transmembrane events，DNA replication，recombination and repair，etc. Besides，the mutant strain Cgasg1?驻 lost the ability of resisting oxidative stress and hyperosmotic stress. These results indicated that CgAsg1p is an essential transcription factor in C. glabrata for various stress responses.