混合碳源对灵芝多糖发酵及其抗肿瘤活性的影响

Effects of Mixed Carbon Sources on Production and Antitumor Activity of Ganoderma lucidum Exopolysaccharides by Submerged Culture

摘要:

为了高效生产活性灵芝多糖，以葡萄糖液体发酵的基本碳源，同时添加两种灵芝多糖的主要单糖组分——半乳糖和甘露糖，研究混合碳源对灵芝生长、多糖合成及其抗肿瘤活性的影响。结果表明，以不同的混合碳源作为灵芝液态发酵的碳源，发现不同比例的单糖碳源对灵芝多糖产量和生物量影响较小。不同的灵芝多糖剂量对皮肤基底癌细胞A431和人乳腺癌细胞MDA-MB-231的增殖均有抑制作用，碳源比例对灵芝多糖活性影响较大。葡萄糖和半乳糖以质量浓度比1∶1作碳源时抑制率可达到50%~60%；葡萄糖和半乳糖质量浓度比1∶2作碳源时抑制率可达到75%~85%；葡萄糖和甘露糖质量浓度比1∶1作碳源时抑制率可达到60%~65%；葡萄糖和甘露糖质量浓度比1∶2作碳源时抑制率可达到80%~85%；葡萄糖、半乳糖和甘露糖质量浓度比1∶1∶1作碳源时抑制率可达到80%~85%。初始培养基中半乳糖和甘露糖所占比例大有利于灵芝多糖抗肿瘤活性的发挥。

Abstract:

To enhance production and activity of G. lucidum exopolysaccharides（EPS），a mixed carbon source containing glucose，galactose and mannose was applied and effects of mixed carbon sources on cell growth，EPS composition and antitumor activity were studied. Results showed that dry cell weight and EPS production were slightly affected by the ratio and type of monosaccharides. Anti-tumor activity analysis of G. lucidum EPS indicated that the raise of EPS concentration increased its biological activity. The inhibition reached up to 50%~60% with the ratio of glucose to galactose 1∶1 as carbon source，75%~85% with the ratio of glucose to galactose 1∶2，60%~65% with the ratio of glucose to mannose 1∶1，80%~85% with the ratio of glucose to mannose 1∶2 and 80%~85% with the same concentrations for three monosaccharides. The high ratio of galactose or mannose in culture medium was beneficial to the anti-tumor activity of EPS against the growth of A431and MDA-MB-231 cells.