Colletotrichum lini ST-1静息细胞转化DHEA制备7α，15α-diOH-DHEA的工艺

Bioconversion of Dehydroepiandrosterone to 3β，7α，15α-Trihydroxy-5-Androsten-17-One by Colletotrichum lini ST-1 Resting Cells

摘要:

利用亚麻刺盘孢（Colletotrichum lini ST-1）静息细胞转化去氢表雄酮，制备三羟基雄甾烯酮，通过单因素优化确定了最佳摇瓶转化条件：细胞质量浓度为12 g/L，pH值为6.5，装液量为30 mL/250 mL，转速为220 r/min，温度为30℃。在上述条件下，底物投料质量浓度为10 g/L时，转化48 h，产物摩尔得率为38.5%，较生长细胞提高了21.0%。在上述工作基础上，尝试了静息细胞连续批次转化，最终通过两批次的连续转化，在底物累计投料质量浓度为18 g/L时，转化78 h，产物的累积质量浓度高达12.1 g/L，产物摩尔得率可达60.5%。

Abstract:

The bioconversion of dehydroepiandrosterone（DHEA） to 3β，7α，15α-trihydroxy-5- androsten-17-one by Colletotrichum lini ST-1 resting cells was investigated. The optimal transformation parameters in flasks were cell concentration 12 g/L，pH 6.5，liquid volume 30 mL/250 mL，rotational speed 220 r/min and temperature 30 ℃，which resulted in a molar product yield of 38.5% for 7α，15α-diOH-DHEA that was 20.1% higher than that from growing cells. Based on these conditions，two-batch bioconversion for 78 h with resting cells at a total substrate concentration of 18 g/L resulted in a concentration of 12.1 g/L and a molar product yield of 60.5% for 7α，15α- diOH-DHEA.