深层发酵灵芝胞内多糖的组成与结构分析

Structure Features and Monosaccharide Composition of the Intracellular Polysaccharide from Submerge-Cultured Ganoderma lucidum

摘要:

测定了深层发酵灵芝胞内多糖的分子量和单糖组成，首次详细分析了深层发酵灵芝胞内多糖的结构特性，为深入研究深层发酵灵芝多糖的生物活性及其构效关系奠定了基础。经单糖组成分析和光谱初步解析，灵芝胞内多糖是由5种单糖组成，其摩尔比为葡萄糖∶半乳糖∶甘露糖∶阿拉伯糖∶木糖=83.75∶4.76∶4.15∶2.04∶5.30；通过IR、GC-MS、HPAE-PAD、NMR等分析其是以α-（1→4）位键合吡喃葡萄糖主链为主，以T-Glcp为主要的残基，同时存在半乳糖、甘露糖、阿拉伯糖和木糖的分支残基。

Abstract:

The molecular weight and monosaccharide composition of intracellular polysaccharide from submerge-cultured Ganoderma lucidum（GLP） was investigated in this study，whose structure features was also thoroughly studied for the first time. This work could build a solid theoretical foundation for the further research on the biological activity and structure-activity relationship of GLP. The components of intracellular GLP were preliminary determined as glucose，galactose，mannose，arabinose and xylose with a molar ratio of 83.75∶4.76∶4.15∶2.04∶5.30 using monosaccharide composition analysis and spectrum analysis. The structure feature was confirmed with a backbone of 1，4-α-glucan and main terminal sugar residues of T-Glcp，and other branch residues containing galactose，mannose，xylose and arabinose by characterization of IR，GC-MS，HPAE-PAD and NMR，respectively.