茯苓多糖对2型糖尿病小鼠肾组织抗氧化能力及Bax、Bcl-2蛋白表达影响

Effect of Pachymaran to the Antioxidant Capacity and Bax, Bcl-2 Protein Expression of Renal Tissue in Mice with Type II Diabetes

摘要:

研究探讨茯苓多糖（WRP）对2型糖尿病（NIDDM）小鼠肾组织抗氧化能力及Bax、Bcl-2蛋白表达影响。采用高糖高脂饲料＋小剂量链脲佐菌素（STZ）方式诱导NIDDM动物模型，然后将动物分成正常对照组、模型对照组、WRP灌胃组、罗格列酮灌胃组，药物连续灌胃42 d，检测小鼠肾组织抗氧化能力以及Bax和Bcl-2蛋白的表达。结果表明，WRP能使NIDDM小鼠肾组织中超氧化物岐化酶、谷胱甘肽过氧化物酶水平明显升高，丙二醛的水平明显降低；即WRP能增强机体肾脏的抗氧化性，降低脂质过氧化，保护自由基介导的氧化损伤，减轻糖尿病对肾脏的损害。WRP能抑制NIDDM小鼠肾组织中Bax基因过多表达；使糖尿病状态下肾组织细胞凋亡趋势受到抑制，对糖尿病肾病具有一定预防作用。

Abstract:

The effect of polysaccharides from Wolfiporia cocos （Schw.） Ryv. & Cilbn（WRP）to the antioxidant capacity and protein Bax，Bcl-2 gene expression of renal tissues in mice with type II diabetes were studied. Non insulin dependent （type II） diabetes mellitus （NIDDM） was induced by the combination of high-carbohydrate/high-fat diet-fed and low dose of streptozotocin （STZ）. Mice were randomly assigned to four treatment groups：the control group，the NIDDM group，the group fed WRP diet and the group fed rosiglitazone diet. The antioxidant capacity and the expression of Bax and Bcl-2 in renal tissues of mice were investigated. The superoxide dismutase （SOD） and glutathione peroxidase （GPx） levels in NIDDM mice were increased while the malondialdehyde （MDA） level was decreased after fed with WRP，which indicated that WRP could protect kidney from the damage caused by diabetes by improving the antioxidation activity of kidney，reducing lipid peroxidation and preventing free radical induced oxidative stress. WRP could restrain excessive expression of bax gene in NIDDM mice and inhibit apoptosis of renal cells，which indicated preventive of diabetic nephropathy.