耐有机溶剂α-葡萄糖苷酶的补料分批发酵研究

Enhanced Production of Solvent-Stable Alpha Glycosidase from Bacillus licheniformis JXC-1 by an Optimal Feeding Strategy

摘要:

作者研究了Bacillus licheniformis JXC-1在3 L发酵罐上产耐有机溶剂α-葡萄糖苷酶的关键因素，结果表明：pH恒定7.0，初始糖质量浓度25 g/L，搅拌转速600 r/min为最适产酶条件，酶活由104.6 U/L提高到了444.7 U/L。通过4种补料策略对其进行补料分批发酵研究，得出：4~5 h内补料速度为2.25 mL/h，5~6 h内补料速度为6.75 mL/h，6~7 h内补料速度为9 mL/h，7~8 h内补料速度为15 mL/h补加麦芽糖与蛋白胨效果最好，酶活达到872.5 U/L。

Abstract:

In previous work，we screened a strain（Bacillus licheniformis JXC-1） producing solvent-stable（10% N，N-Dimethylformamide，short for DMF） α-glucosidase from several different soil samples. In this work，we attempted to determine the key factors for the production of α-glucosidase and optimize the feeding strategy in a 3-L fermenter. Results showed that the enzyme activity reached 444.7 U/L with the optimal condition（pH 7.0，the initial maltose concentration 25 g/L，and agitation speed 600 rpm）. The strategy of feeding maltose and tryptone at a rate of 2.25 mL/h（4-5 h），6.75 mL/h（5-6 h），9 mL/h（6-7 h） and 15 mL/h（7-8 h） significantly increased the production of α-glycosidase from 444.7 U/L to 872.5 U/L.