菊糖抗氧化活性及其机理

Studies on the Antioxidant Activity of Inulin and Its Mechanism

摘要:

通过测定菊糖的还原能力、Fe2+螯合能力，以及DPPH自由基清除能力等方法，评价菊糖的抗氧化能力，并采用Caco-2细胞模型探讨其抗氧化机理。3种抗氧化活性实验结果表明，菊糖具有良好的抗氧化活性。与阳性对照组比较，菊糖对H2O2诱导的Caco-2细胞氧化损伤具有显著的保护作用。当菊糖质量浓度为0.8 mg/mL时，Caco-2细胞中过氧化氢酶、谷胱甘肽还原酶，以及谷胱甘肽过氧化物酶的活力，分别提高了35.8%，47.6%和61.2%。菊糖主要通过提高细胞内抗氧化酶的活力发挥其抗氧化作用。

Abstract:

The antioxidant activity of inulin was evaluated by the studies of the reduction ability，Fe2+-chelating activity and DPPH free radical scavenging activity. The mechanism of its antioxidant activity was investigated using the Caco-2 cell monolayer model. Three different antioxidant activity assays showed that inulin exhibited good antioxidant activity. A significant protective effect of inulin on H2O2 induced oxidative stress in Caco-2 cells was observed in comparison with the positive controls. With the treatment of 0.8 mg/mL insulin，the activities of superoxide dismutase，catalase and glutathione peroxidase were increased by 35.8%，47.6%，and 61.2%，respectively. The antioxidant activity of inulin takes effect mainly by activing the intracellular antioxidant enzymes.