肠炎沙门氏菌gDNA标准物质的研究

Study on the Reference Materials of Salmonella enteritidis Nucleic Acid

摘要:

摘要： 作者对制备肠炎沙门氏菌核酸标准物质（gDNA）进行初探，共制备200支样品，每支约含有肠炎沙门氏菌gDNA 1 μg。方差分析结果显示，F值为0.835 7，小于F0.05（14，30）=1.68，表明在95%显著性水平时，样品的gDNA含量均匀；稳定性试验表明样品在-20 ℃下保存12个月，gDNA含量维持在1 μg/支，0.8 g/dL的琼脂糖凝胶电泳的目标条带清晰，以invA基因为引物的PCR反应产物电泳条带清晰，样品均具有较好的稳定性。

Abstract:

The preparation of nucleic acid reference materials of Salmonella Enteritidis（gDNA） was carried out in this study. A total of 200 samples were prepared，each of them contained 1 μg of Salmonella Enteritidis gDNA. Variance analysis results showed that F value was 0.835 7