氧化铝对鸭蛋中磷脂酰乙醇胺的吸附过程研究

Studies on the Adsorption Process of Phosphatidylethanolamine in Egg Yolk Adsorbed onto Alumina

摘要:

磷脂在食品和医药行业应用广泛。在磷脂的分离纯化方法的研究中,吸附法是一种应用较普遍的方法。为了更好的分离和纯化磷脂单体,作者利用高效液相色谱法考察了吸附法中吸附剂氧化铝静态吸附磷脂酰乙醇胺的动力学规律和热力学规律。结果表明:氧化铝在鸭蛋黄磷脂/甲醇溶液中吸附磷脂酰乙醇胺的行为符合二级动力学模型,吸附速率主要由分子内部扩散控制,但它并不是唯一的控制因素。等温吸附过程更符合Langmuir方程,吸附过程为熵驱动的放热、熵增的自发过程。

Abstract:

Phospholipids have been widely used in the food and pharmaceutical industries. The adsorption is generally employed to separate and purify phospholipids. The static adsorption of phosphatidyl ethanolamine in egg yolk onto alumina was investigated and the kinetics and thermodynamics of the adsorption were studied using the high performance liquid chromatography. The fit model of the adsorption in phospholipids/methanol solution was the second-order kinetic model, where the internal diffusion was the key control of the adsorption but not the only one. The isothermal adsorption can be well explained using Langmuir Adsorption Isotherm. The adsorption process was a spontaneous exothermic process resulted from the increase in the systems entropy.