夏季室外光生物反应器培养Chlorella sorokiniana

Outdoor Chlorella sorokiniana Cultivation Using Photobioreactor in Summer

摘要:

搭建了一个6 L容积曝气型垂直柱光生物反应器,在6-8月期间置于室外屋顶运行,旨在考察夏季室外培养微藻Chlorella sorokiniana的可行性。室外培养时,若pH超过8.5,微藻会出现明显絮凝沉淀现象。太阳光入射到反应器的高达(1 700±137) ?滋mol/(m2·s)的光照强度带来的光抑制作用及随之引起的超过48 ℃的高温,对微藻细胞的损伤造成了室外培养的抑制现象。当培养液温度超过40 ℃时,Chlorella sorokiniana几乎不能生长。在反应器周围放置金属镀层反射隔热膜反射板,可以减少(42.21±4.7)%的光照强度,使培养液温度下降2~4 ℃,但晴天时光照强度仍然可达900 ?滋mol/(m2·s)以上,光抑制作用不能很好被解除。为实现微藻在室外反应器中可持续地快速增长,仍需进一步采取措施减弱光照强度。

Abstract:

Aimed to investigate the feasibility of growing Chlorella sorokiniana outdoor in summer,a 6 L aerated vertical column photobioreactor was performed from Jun to Aug on the rooftop. When cultivated outdoor,algae auto-flocculation will happen heavily once pH was over 8.5. The photoinhibition caused by high incident light intensity(1 700±137 ?滋mol/(m2·s))and the algal cells damage resulted from high temperature(48 ℃) lead to inhibition of algal growth. Chlorella sorokiniana is not able to grow well when temperature is over 40 ℃. The installation of reflectors,which covered by reflective thermal insulation mental coatings,could decrease light intensity by 42.21±4.7%,resulting in 2~4 ℃ temperature decrease. However,light intensity still could be more than 900 ?滋mol/(m2·s) on sunny days,making photoinhibition hard to be relieved. To achieve faster algal growth as well as continuous cultivation outdoor,more effort should be made to moderate the light intensity.