近红外荧光探针及其在免疫分析中的应用

Near Infrared Fluorescent Probes and Their Applications in Immunoassay

摘要:

综合归纳了有机荧光分子、量子点、稀土配合物及单壁碳纳米管共4类重要近红外荧光标记探针的性质、特征,及其在光学性能改进、信号增强等方面的最新发展,分析评述了其在环境污染物及临床诊断标志分子免疫分析中的应用,展望了基于该类探针的免疫层析法在食源性致病菌快速检测中的应用潜能。相对于发射光光谱位于紫外及可见光区的信号分子,近红外荧光探针因其具有信噪比高、组织穿透力强、对基体损伤小等突出的优势,而在生物分析领域备受瞩目。随着化学合成技术的不断发展及新型荧光材料的持续发掘,近年来近红外荧光探针日益丰富,并在无损分析、免疫检测和生物造影等领域被广泛应用。

Abstract:

Owing to the distinguished superiority of lower background noises,deeper penetrating capacity and less destructiveness to biomatrix over UV and visible fluorophores,near-infrared fluorescent probes(NIFPs) have gaining more and more attentions for analytical applications to date. With continuous research efforts in chemical synthesis and exploration of novel fluorescent materials,the number of NIFPs applicable for biological systems has grown substantially and widely applied in fields such as non-destructive detection,immunoassay and bioimaging in recent years. In this review,the properties,characterization and recent progresses in improvements of optical properties and signal intensity of 4 types of vital NIFPs(i.e. organic fluorophores,quantum dots,rare earth compounds and single-walled carbon nanotubes) were summarized. The applications of such important NIFPs in immunological analysis of environmental contaminants and clinically important biomarkers were also elaborated. Moreover,the potential of NIFPs-based immunochromatography technique adaptable for rapid detection of foodborne pathogens was also forecasted.