血红密孔菌Pycnoporus sanguineusSYBC-L12固态发酵小麦秸秆产漆酶及其对小麦秸秆的降解作用

Laccase Production under Solid-State Fermentation of Wheat Straw and Its Degradation by Pycnoporus sanguineus SYBC-L12

摘要:

以小麦秸秆为基本培养基质,漆酶活力为指标,对其培养条件--包括碳源、氮源、起始含水量和秸秆粒度进行了初步的优化,得到了较佳产酶条件。在此过程中测定了发酵后还原糖的质量分数,探究了白腐菌在小麦秸秆降解过程中的作用。另外,测定了添加氮源发酵后的麦草秸秆与未发酵麦草秸秆的组成,并进行了比较。

Abstract:

Wheat straw was used as basic substrate and supporter for laccase production under solid-state fermentation(SSF) in this essay and optimized its best suitable cultural conditions for laccase production including carbon and nitrogen sources,initial moisture and physical size of wheat straw. Meanwhile,the reduced sugar generated during the SSF was measured and the function of the white-rot fungi in wheat straw degradation. Furthermore,we compared the consistence of wheat straw before and after SSF containing nitrogen sources.