超高压处理对蛹虫草汁纤维素酶活性的影响

Effect of Ultrahigh Pressure Processing on the Activities of Cellulose in C. militaris Juice

摘要:

为了明确超高压加工技术对纤维素酶活性的影响，分别研究了压力、保压时间、温度、乙醇体积分数、加压次数和pH值等对蛹虫草汁中纤维素酶的羧甲基纤维素酶活性（CMC）和滤纸酶活（FPA）的影响。结果表明，纤维素酶的最适压力、保压时间、温度、乙醇体积分数、pH值和加压次数分别为：400 MPa、10 min、35 ℃、30 %，pH 4.0~7.0，加压1次，在最适条件下，CMC酶活比处理前分别提高了82.07%、52.41%、87.22 %、54.48%、67.52%和66.67 %；FPA酶活比处理前分别提高了97.37%、61.4 %、69.4 %、61.4%、1.31%和33.91 %。因此，利用超高压处理能有效提高蛹虫草汁中的纤维素酶活性。

Abstract:

In order to clarify the effects of ultrahigh pressure processing（UHP） on the activities of cellulose, the CMC and FPA enzyme activities of cellulose in C. militaris juice were studied by pressure, holding time, temperature, ethanol concentration, times of pressure and pH. The result showed that the optimal pressure, holding time, temperature, ethanol concentration, times of pressure and pH of the enzyme were 400 MPa, 10 min, 35 ℃, 30 %, pH 4.0-7.0 and pressure for 1 times, respectively. Under the optimum conditions, the CMC enzyme activity were enhanced by 82.07%, 52.41%, 87.22 %, 54.48%, 67.52% and 66.67 %, and the FPA enzyme activity was enhanced by 97.37%, 61.4 %, 69.4 %, 61.4%, 1.31% and 33.91 %, respectively. The experiment illustrated that the ultra-high pressure treatment could enhance the activity of cellulose in C. militaris juice.