羊奶中挥发性成分顶空固相微萃取条件的优化

Optimization of Head Space Solid Phase Micro-Extraction Conditions for Volatile Components in Goat Milk

摘要:

采用顶空固相微萃取结合气相色谱检测羊奶中挥发性成分，以出峰数和峰面积为指标，对影响固相微萃取法提取羊奶中挥发性成分的萃取头、萃取温度、加盐量、萃取时间和解析时间5个因素进行优化。结果表明，选用CAR/PDMS为萃取头、NaCl添加量为0.3 g/mL、萃取温度为70 ℃、萃取时间为90 min、解析时间为5 min时，可最大限度地提取羊奶中挥发性成分。再以最优条件萃取羊奶中挥发性成分，经GC/MS分析检测，共检测出42种挥发性成分，其中酸性成分最多，有14种，其次是酯类、烷烃、芳香族、杂环、醇和醛酮，分别有6、5、5、5、4和3种。

Abstract:

Head Space Solid phase micro-extraction（HS/SPME） coupled with gas chromatography（GC） were used for the determination of volatile compounds in goat milk. HS/SPME experimental parameters, such as SPME fibre, extraction temperature, NaCl concentration, extraction time and desorption time were optimized using the number and area of peaks as index. The optimal extraction efficiency for goat milk was achieved when sample was headed at 70 ℃ for 90 min after the addition of 0.3 g/mL NaCl, extracted by a carboxen/polydimethylsiloxane（CAR/PDMS） fibre, and than the fibre was transferred to a GC injection port for thermal desorption at 250 ℃ for 5 min. As a result, 42 kinds of volatile components were detected that including acids（14 kinds）, esters（6 kinds）, hydrocarbons（5 kinds）, alcohols（4 kinds）, aldehydes（3 kind）, aromatic（5 kinds） and heterocyclic（5 kinds）.