Chitinibacter sp. GC72的筛选鉴定及其几丁质降解产物研究

Isolation and Characterization of a Chitin-Degrading Strain Chitinibacter sp. GC72 and Identification of its Chitinase Degradation

摘要:

为向几丁质酶资源的开发利用提供研究基础，作者筛选获得了一株具有高产几丁质酶活性的细菌。经形态、生理生化及分子生物学研究手段鉴定表明该菌属奈瑟菌科，Chitinibacter属，命名为Chitinibacter sp. GC72。经1 L发酵体系检测，其粗酶液酶活可达2 835 U/g。利用薄层层析法、液相色谱法和质谱法对其酶解几丁质产物进行了分析鉴定，结果表明产物主要为N-乙酰-D-氨基葡萄糖。最后利用该酶进行了多次酶解实验，证实投入3.44 U酶量经27 h转化可降解200 mg几丁质至159 mg N-乙酰-D-氨基葡萄糖，收率为79.6%。

Abstract:

A novel chitin-degrading aerobe, Chitinibacter sp. GC72, was isolated from a soil sample from Nanjing, and was proved to have high chitinolytic activity. The enzyme activity could reach to 2 835 U/g at 72 h of culture time, and one liter fermentation liquid contained 910 U chitinase. Its chitinase hydrolyzate, further investigated by TLC、UHPLC、Q-TOF-MS, was confirmed as GlcNAc. In the experiments of chitin hydrolyzation, the GlcNAc yield was 79.6% by using 3.44 U chitinase to hydrolyze chitin（200 mg） in 27 h.