温敏型透明质酸纳米靶向药物传递系统

Study of Temperature Sensitive Nano Drug Delivery System Based on Hyaluronic Acid

摘要:

通过"Click"反应连接透明质酸与普朗尼克F127, 制备两亲性材料(HA-F127), 其结构通过1H NMR确认, 接枝率为13%。材料在水溶液中可自组装形成纳米粒子, 并能够包载抗肿瘤模型药物阿霉素。体外药物释放实验显示, 其具有温度敏感释药特性。透明质酸可识别肿瘤细胞外过度表达的CD44受体, 纳米粒子可靶向传递药物进入肿瘤细胞, 且表现出良好的治疗效果。

Abstract:

An amphiphilic material(HA-F127) was prepared by conjugating hyaluronic acid(HA) and pluronic(F127) via "Click" chemistry. The chemical structure of HA-F127 was identified through 1H NMR spectrum, and the degree of substitution was 13%. HA-F127 could self-assemble into nanoparticles in aqueous solution and load model anticancer drug doxorubicin. In vitro drug release assay showed the nanoparticle behaved temperature sensitive drug release profile. Since hyaluronic acid could recognize CD44 receptor, which over-expressed on cancer cell surface, the nanoparticles could targetedly deliver drug into cancer cells and obtain promoted therapeutic effect.