高渗胁迫对光滑球拟酵母转录组的影响

Transcriptome Analysis of Torulopsis glabrata Under Hyperosmotic Stress

摘要:

采用表达谱芯片技术, 研究了光滑球拟酵母细胞在不同渗透压条件下(860、1 765、2 603 mOs mol/kg和3 324 mOs mol/kg)的转录水平变化情况。结果表明, 在高渗透压条件下, 表达量大幅度提高的基因主要包括一些与细胞壁、细胞膜合成相关的基因, 以及细胞膜上的信息素蛋白质基因。中心代谢途径(即糖酵解途径、三羧酸循环和氧化磷酸化途径)整体表现为显著上调, 特别是参与ATP和NADH合成的相关基因。此外, 对可能作为相容性溶质的氨基酸, 如谷氨酸、脯氨酸、精氨酸的合成代谢途径的分析表明, 脯氨酸和谷氨酸合成途径中的酶转录水平均有一定程度的提高, 但是幅度不大; 而精氨酸分解途径中的尿素羧化酶转录水平发生显著下调, 其转录水平下降至1/100以下。

Abstract:

Gene expression profiles of Torulopsis glabrata at different osmolarity(860, 1 765, 2 603 mOs mol/kg and 3 324 mOs mol/kg) were investigated by microarray. Significantly upregulated genes were mainly involved in regulation of cell wall, cell membrane and pheromone-regulated membrane proteins. The genes in central metabolic pathway, such as glycolysis, Krebs cycle and oxidative phosphorylation pathway, were mainly upregulated, especially the genes associated with ATP or NADH formation. Futhermore, the genes associated with potential key compatible solutes, such as proline, arginine and glutamate synthesis pathway were upregulated, and urea carboxylase which involved in arginine catabolism was drasticly downregulated to only about 1/100.