咖啡因影响肠道甜味感受和机体葡萄糖代谢平衡

Caffeine Affects Intestinal Sweet Perception and Glucose Homeostasis

摘要:

用质量浓度为0.3 g/L的咖啡因溶液对6周大ICR小鼠进行21 d的长期慢性暴露, 暴露完成后采用实体显微镜观察、western-blotting、ELISA及血糖测定等技术, 分别检测了咖啡因对小鼠肠道小肠绒毛形态, 甜味感受和味觉信号转导蛋白质表达, 胰岛素和GLP-1、血糖耐受性的影响。与对照组相比, 咖啡因长期刺激增加了小鼠小肠绒毛高度, 降低了隐窝深度, 从而显著增加了绒毛绒腺比; 咖啡因长期暴露增加了小鼠肠道中甜味受体蛋白质T1R2和T1R3的表达, 降低了味觉信号传导分子α-gustducin表达, 然而对SNAP25的表达无显著影响; 此外, 咖啡因长期暴露显著增加了小鼠肠道对Insulin和GLP-1的释放敏感度, 因此也显著性增强了小鼠的血糖耐受性。结果表明, 咖啡因暴露可以改变小鼠小肠内的甜味感受通路和对机体血糖动态平衡产生影响。

Abstract:

Postnatal 6 week ICR mice were exposed to 0.3 g/L caffeine solution for 21 days. After exposure, the influence of caffeine on intestinal villi morphology, expression of sweet receptors and taste signal transduction protein, release of insulin and GLP-1, glucose tolerance of mice were detected respectively by using the methods of stereomicroscope observation, western-blotting, ELIASA and blood glucose measurement technology. Compared with the control, the height of intestinal villi increased, the depth of crypt of intestinal villi decreased, thereby the ratio of villi and crypt of mice after long-term chronic caffeine exposure. Besides, long-term bitter taste exposure of mice increased the expression of TIR2 and TIR3, but decreased the expression of taste signal transduction molecule α-gustducin, however had no remarkable effect on the expression of SNAP 25. In addition, long-term caffeine exposure significantly increased the release sensitivity of insulin and GLP-1 of mice intestine, so notably increased the blood glucose tolerance of mice. The results indicated that caffeine exposure could alter the sweet perception pathway in intestine, and affect the blood glucose homeostasis of mice.