降胆固醇乳酸菌的筛选及其对小白鼠的不良影响

Screening of Cholesterol-Reducing Lactobacillus and Adverse Effects in Mice

摘要:

对分离自内蒙古家庭传统发酵乳制品中的14株乳杆菌进行体外筛选。结果表明, 试验菌株均有降解MRS-CHOL培养基中胆固醇的能力, 对胆固醇的去除率在6.84％～42.97％。其中N59-1试验菌株对胆固醇的去除率为42.97％(P<0.05)。经生理生化特性和16S rDNA部分序列分析, 菌株N59-1与标准菌株L.plantarum的同源性为99.7％, N59-1可被判定为L.plantarum。30 d喂养试验结果表明, 各组小鼠的组织生长发育及血液理化指标等与对照组相比无显著差异(P>0.05)。

Abstract:

A total of 14 strains were screened that they initially isolated from homemade traditional fermented milk, which was sourced from Inner Mongolia, China. The results showed that all strains could remove cholesterol from culture medium of MRS-CHOL(P<0.05), and the percentage of Cholesterol-degrading rate ranged from 6.84% to 42.97%. Among them, strain N59-1 was selected after screening, which was based on their cholesterol removal abilities. The cholesterol removal rate was 42.97％. The strain N59-1 with the highest level of cholesterol removal from the media was identified using physiological and biochemical characteristics and 16S rDNA sequences, the homology of N59-1 with L. plantarum was 99.7%. As a result N59-1 was named L. plantarum. 30 days feeding test results showed that the organ growth and blood biochemical index of mice in each group had no significant difference compared with the control group(P>0.05).